monoclonal anti human egf neutralizing antibody  (R&D Systems)

Journal: PLoS ONE

Article Title: Control of Angiogenesis by Galectins Involves the Release of Platelet-Derived Proangiogenic Factors

doi: 10.1371/journal.pone.0096402

Figure Lengend Snippet: A) WPs were stimulated with Gal-1 (5 µM), Gal-3 (5 µM) or Gal-8 (1 µM) for 5 min. The release of angiogenic factors was determined using a commercial array kit. Release of each molecule induced by individual galectins is expressed as fold increase over unstimulated controls (n = 2). B) HMEC-1 were preincubated with anti-EGFR antibody (20 µg/ml) or irrelevant IgG during 30 min. Then, cells were incubated for 18 h with EGF (20 ng/ml) or with supernatants from unstimulated or galectin-stimulated platelets. Endothelial cell proliferation and tube formation were analyzed (n = 3; * P <0.05 vs. unstimulated, # P <0.05 vs. without neutralizing antibody (Ab)).

Article Snippet: Rabbit anti-human EGF neutralizing antibody and control IgG were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Incubation